The internal webserver of Tryton is only for development purposes, so you need something like uwsgi or gunicorn to run Tryton. When you have that running, you can create a systemd service file which starts it all automatically on boot.
Personally I prefer gunicorn it’s a more general purpose wsgi server.
None, but the internal werkzeug wsgi server of Tryton is NOT to be used in production and only for development. In order to test properly in a more automated manner just install Gunicorn and a systemd file to start Tryton on boot. The internet is full of examples how to do this.